液肥

Hoagland solution

half-strength Hoagland solution (original)

| Stock | Volume (mL) |
| :--------------- | :---------: |
| DW | 1000 |
| A | 5 |
| B | 5 |
| C | 5 |
| E | 2.5 |
| F | 0.5 |
| | |
| Adjust to pH 6.0 | |

half-strength Hoagland solution (for mycorrhizal)

| Stock | Volume (mL) |
| :--------------- | :-----------------: |
| DW | 1000 |
| A | 5 |
| B | 5 |
| C | 1 (final 100 µM Pi) |
| E | 2.5 |
| F | 0.5 |
| | |
| Adjust to pH 6.0 | |


Pi濃度をstock Cの加える量で変える場合、K⁺濃度が変化するのでstock D（K₂SO₄）でK⁺濃度を調節する。K⁺のモル数に注意する。
When it is nessesary to change concentrations of Pi in stock C, a concentration of K⁺ should be adjusted by adding stock D.

Stock solutions

stock A

| Reagent | Weight (g) | Conc (mM) |
| ---------------------------------------------------------- | :--------: | :-------: |
| KNO₃ (MW 101.11) | 2.528 | 500 |
| Ca(NO₃)₂・4H₂O (MW 236.15) | 5.904 | 500 |
| DW | 50 mL | |


stock B
|Reagent|Weight (g)|Conc (mM)|
|---|:---:|:---:|
|MgSO₄・7H₂O (MW 246.47)|2.465|200|
|DW|50 mL||

stock C
|Reagent|Weight (g)|Conc (mM)|
|---|:---:|:---:|
|MKH₂PO₄ (MW 136.08)|0.680|100|
|DW|50 mL||
K₂HPO₄と間違わないように注意

stock D
|Reagent|Weight (g)|Conc (mM)|
|---|:---:|:---:|
|K₂SO₄ (MW 174.26)|1.743|200|
|DW|50 mL||

stock E
|Reagent|Weight (g)|Conc (%)|
|---|:---:|:---:|
|Fe(III)-EDTA (MW 421.09)|1|2|
|DW|50 mL||

stock F
|Reagent|Weight (g)|Conc (g/L)|
|---|:---:|:---:|
|H₃BO₃ (MW 61.83)|0.143|0.5|
|MnCl₂・4H₂O (MW 197.90)|0.090|0.5|
|ZnSO₄・7H₂O (MW 287.56)|0.011|0.05|
|CuSO₄・5H₂O (MW 249.68)|0.004|0.02|
|Na₂MoO₄・2H₂O (MW 241.95)|0.00126|0.01|
|DW|50 mL||
※Zn, Cu, Moは加える量が微量なため、あらかじめ0.1g/mlの溶液を作成し、それぞれ110 µl, 40 µl, 12.6 µl加える。

微量元素ストック液の作り方
1. Weigh 0.143 g of H₃BO₃ and add it in a 50 or 100 mL beaker.
2. Weigh 0.090 g of MnCl2・4H₃O and add it in the same beaker.
3. Add approximately 40 mL of distilled water in the beaker and dissolve the reagents well.
4. Prepare concentrated Zn, Cu and Mo solutions as below. Dissolve 0.1 g of ZnSO₄・7H₃O in 1 mL distilled water in a microtube. Dissolve 0.1 g of CuSO4・5H2O in 1 ml distilled water in a microtube. Dissolve 0.1 g of Na2MoO4・2H2O in 1 ml distilled water in a microtube.
5. Add 110 µL of concentrated Zn solution, 40 µl of concentrated Cu solution, 12.6 µl of concentrated Mo solution into the B and Mn mixture prepared in the step 3
6. Add distilled water to the mixture in a measuring cylinder to make 50 ml of stock F.
7. Store at 4℃.